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目的体外检测骨髓基质细胞分泌物对PC12细胞的活性作用,并探讨其产生的可能机制。方法收集培养至第4代第7天的SD大鼠MSCs培养上清,按不同的体积百分比浓度加入到PC12细胞培养体系中,在倒置相差显微镜下观察1 d和4 d的细胞形态学改变;用丙二酸钠对PC12细胞造成氧化应激损伤,同时加入不同体积百分比浓度的MSCs培养上清,采用MTT法测定24 h后的细胞活性。结果有突细胞/总细胞数、最长突起长度随培养时间及MSC培养上清体积百分比的增加而增加;PC12细胞氧化损伤后,加入一定浓度MSC培养上清组的PC12细胞活性较未加入组增高,差异有显著性。结论MSCs能够合成和分泌具有神经营养活性的物质,该物质能诱导PC12细胞分化并减轻氧化应激对PC12细胞的损伤。
Objective To detect the activity of bone marrow stromal cells secreting PC12 cells in vitro and to explore the possible mechanism. Methods The culture supernatant of SD rat MSCs cultured on the 7th day of passage 4 was collected and added to the culture system of PC12 cells at different volume percentages. The morphological changes of cells were observed under inverted phase contrast microscope at 1 and 4 days. Oxidative stress damage to PC12 cells was induced by sodium malonate, and the supernatant of MSCs with different volume percent concentration was added. The cell viability was measured by MTT assay after 24 h. The results showed that the number of cells / total cells, the longest protrusion length with the culture time and the volume of MSC culture supernatant increased; PC12 cells after oxidative injury, adding a certain concentration of MSC culture supernatant PC12 cell activity than the group did not join Increased, the difference was significant. Conclusion MSCs can synthesize and secrete neurotrophic substances, which can induce the differentiation of PC12 cells and reduce the damage of oxidative stress on PC12 cells.