目的利用噬菌体表面展示技术,从肺癌T7噬菌体展示文库中筛选MUC1蛋白抗原的模拟表位。方法以MUC1单克隆抗体为靶分子,对肺癌T7噬菌体文库进行淘洗筛选,得到的阳性克隆进行测序并推导其氨基酸序列,对得到的阳性噬菌体克隆进行鉴定。结果经3轮淘选,得到20个阳性克隆。测序获得AAPDFRP、SAPDDRP 2种氨基酸序列,对MUC1单抗的抑制率均在50%以上,与肺癌血清结合特异性较高,此2种蛋白序列可模拟MUC1蛋白抗原表位。结论通过噬菌体展示技术成功筛选到MUC1蛋白的模拟表位序列XAPDXRP(X为任意氨基酸),对肺癌的早期诊断有一定的参考价值。 Objective To screen mimic epitopes of MUC1 protein antigen from lung cancer T7 phage display library by phage display technique. Methods The MUC1 monoclonal antibody was used as the target molecule to screen the lung cancer T7 phage library. The positive clones were sequenced and their amino acid sequences were deduced. The positive phage clones were identified. Results After 3 rounds of panning, 20 positive clones were obtained. The two amino acid sequences of AAPDFRP and SAPDDRP were sequenced. The inhibitory rates of MUC1 monoclonal antibody were both above 50%, which were highly specific to lung cancer serum. These two protein sequences could mimic MUC1 protein epitopes. Conclusion The phage display technique successfully screened the mimic epitope sequence MUC1 XAPDXRP (X is an arbitrary amino acid), which is of certain reference value for the early diagnosis of lung cancer.