论文部分内容阅读
目的:建立大鼠体内落新妇苷的SPE-HPLC分析方法,通过复方银屑灵与单味土茯苓中落新妇苷药代动力学参数的比较,探讨配伍对落新妇苷大鼠体内药代动力学行为的影响。方法:雄性SD大鼠分别灌胃银屑灵、土茯苓提取物后,于不同时间眼眶静脉丛采血,固相萃取(SPE)法处理。采用反相高效液相色谱法,以甲醇-乙腈-0.05%甲酸为流动相梯度洗脱;Phenomenex C18色谱柱,柱温24℃,流速0.8 mL.min-1。结果:落新妇苷在0.266～53.1 mg.L-1线性关系良好(r=0.996);提取回收率为79.0%～89.1%;用非隔室模型估算药物动力学参数t1/2,Cmax,AUC0-t,AUC0-∞,MRT,各参数具有统计学意义。结论:该法可用于落新妇苷的药代动力学研究;配伍可提高落新妇苷在大鼠体内的生物利用度。
OBJECTIVE: To establish a SPE-HPLC method for the determination of astilbin in rats. By comparing the pharmacokinetics of stilbene in Compound Psoriasis and Tuckahoe, the pharmacokinetics of Astilbin in rats The impact of learning behavior. Methods: Sprague - Dawley and Smilax glabra extracts were administered to male Sprague - Dawley rats, and were treated with orbital venous plexus by SPE at different times. The mobile phase was methanol-acetonitrile-0.05% formic acid. Phenomenex C18 column was used with a column temperature of 24 ℃ and a flow rate of 0.8 mL · min-1 using reversed-phase high performance liquid chromatography. Results: The calibration curve was linear between 0.266 and 53.1 mg.L-1 (r = 0.996). The recoveries ranged from 79.0% to 89.1%. The pharmacokinetic parameters t1 / 2, Cmax and AUC0 -t, AUC0-∞, MRT, the parameters have statistical significance. Conclusion: The method can be used to study the pharmacokinetics of astilbin; compatibility can improve the bioavailability of astilbin in rats.