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从人宫颈癌组织中制得高分子DNA,选用NIH/3T3细胞作受体,以含抗新霉素基因的表达载体(PSV-neo)作为抗性选择标记和人宫颈癌DNA进行共转染。转染后的细胞采用G_(418)和低血清进行筛选。其结果获得了形态恶变的转化细胞灶,它能在0.3%软琼脂培养基中生长,在裸鼠体内成瘤,并证实为纤维肉瘤。而对照NIH/3T3细胞却没有这种能力和性能。转化灶DNA能与标记探针Blu-R8起杂交反应。提示人Alu重复序列片段也整合到转染体基因组中。
Preparation of macromolecular DNA from human cervical cancer tissues, selection of NIH/3T3 cells as receptors, and cotransfection of a neomycin-resistant expression vector (PSV-neo) as a resistance selection marker and human cervical cancer DNA . Transfected cells were screened with G_(418) and low serum. As a result, malignant transformed cells were obtained, which grew in 0.3% soft agar medium, became tumorigenic in nude mice, and proved to be fibrosarcoma. Control NIH/3T3 cells did not have this ability and performance. The DNA of the foci can hybridize with the labeled probe Blu-R8. It is suggested that the human Alu repeat fragment is also integrated into the transfectant genome.