手性分离是色谱分离的难点,且常见的手性分离多为正相色谱分离模式。然而,手性物质往往存在于含水体系中,手性化合物的拆分和检测在反相分离模式中具有更好的相容性。分别介绍了手性药物、手性氨基酸的反相色谱分析进展,并综述了手性添加剂在反相色谱手性分离中的应用,对手性分离和分析具有重要的参考意义。 Chiral separation is the difficulty of chromatographic separation, and the common chiral separation is mostly normal phase chromatographic separation mode. However, chiral materials are often found in aqueous systems, and the resolution and detection of chiral compounds have better compatibility in reversed-phase separation mode. The chiral drugs and chiral amino acids were reviewed respectively. The application of chiral additives in the chiral separation of reversed-phase chromatography was reviewed. It is of great significance to chiral separation and analysis.