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目的:四逆散传统汤剂和配方颗粒是在临床中同时存在的两种用药形式,本研究拟对比研究四逆散传统汤剂和配方颗粒的疏肝解郁药效作用,为四逆散配方颗粒的临床使用提供实验依据。方法:昆明种小鼠随机分为空白组、应激模型组、阳性药组、四逆散传统汤剂低、高剂量(600,1 200 mg.kg-1)组、四逆散配方颗粒汤剂低、高剂量(600,1 200 mg.kg-1)组共7组,每组10只。小鼠连续灌胃给药1周,实验第6天时给予小鼠一次性拘束应激18 h,测定血浆丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平。昆明种小鼠随机分为空白组、粉刺杆菌-脂多糖(Propionibacterium acne-LPS)致肝炎模型组、阳性药组、四逆散传统汤剂低、高剂量(600,1 200 mg.kg-1)组、四逆散配方颗粒汤剂低、高剂量(600,1 200 mg.kg-1)组共7组,每组10只。小鼠尾静脉注射20 mg.kg-1热灭活粉刺杆菌生理盐水溶液5 d后,尾静脉注射5μg.kg-1脂多糖生理盐水溶液,5 h测定血浆ALT,AST水平。结果:与正常对照组相比,应激模型组和P.acnes-LPS的血浆ALT均显著增高(43.4±2.0)U.L-1vs(12.2±2.8)U.L-1,P<0.05;(114.9±23.3)U.L-1 vs(18.8±5.2)U.L-1,P<0.05,AST水平也显著增高。与模型组相比,四逆散传统饮片汤剂和配方颗粒均能显著降低应激负荷和粉刺杆菌-脂多糖升高的血浆ALT和AST水平,但是两药药效没有统计学差异。结论:四逆散传统饮片汤剂和配方颗粒均有较好的保肝作用。
Objective: Sini powder traditional decoction and formula particles in the clinical application of the two forms of co-exist in this study to compare the traditional Sini inverse formula and traditional Chinese medicine decoction Shugan Jieyu efficacy of Sini The clinical use of formula particles provides experimental evidence. Methods: Kunming mice were randomly divided into blank group, stress model group, positive drug group, Sini Powder traditional decoction low and high dose (600,1 200 mg.kg-1) group, Sini Powder formula soup A total of 7 groups of low dose and high dose (600,1 200 mg.kg-1) group, 10 rats in each group. Mice were given gavage for 1 week. On the 6th day of the experiment, the mice were given restraint stress for 18 hours. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Kunming mice were randomly divided into blank group, Propionibacterium acne-LPS induced hepatitis model group, positive drug group, Sini Powder traditional decoction low and high dose (600,1 200 mg.kg-1 ) Group. Sini Powder Formula Granule Decoction was given in low and high doses (600,1 200 mg.kg-1) group, with 7 in each group. The mice were intravenously injected with 20 mg.kg-1 heat-inactivated amphotericin saline solution for 5 days, and 5μg · kg-1 lipopolysaccharide saline solution was injected into the caudal vein. The plasma ALT and AST levels were measured 5h later. Results: Compared with the normal control group, the plasma ALT of the stress model group and P. acnes-LPS group was significantly higher (43.4 ± 2.0) UL-1vs (12.2 ± 2.8) UL-1, P <0.05; (114.9 ± 23.3 ) UL-1 vs (18.8 ± 5.2) UL-1, P <0.05, AST levels also significantly increased. Compared with the model group, the Sini Decoction traditional decoction decoction and the formula granule can significantly reduce the stress load and the elevated plasma ALT and AST levels of Acne bacteria - lipopolysaccharide, but there is no statistical difference between the two drugs. Conclusion: Sini Powder traditional decoction and formula particles have better liver protection role.