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目的探讨帕金森病病人中外周血单个核细胞(PBMC)分泌IL-2的能力。方法采用酶联免疫吸附法(ELISA)分别检测体外培养的PBMC上清液、及PBMC接受刀豆蛋白(ConA)刺激后上清液中IL-2含量。结果PDⅠ期患者中未受刺激的PBMC分泌的IL-2显著高于PDⅡ-Ⅲ期患者(P<0.05),接受ConA刺激后,以PDⅡ-Ⅲ期患者的PBMC生成IL-2最低,显著低于正常对照组(P<0.05),刺激后同刺激前相比各组IL-2都有升高,但仅健康对照组刺激前后有显著差异(P<0.05)。结论帕金森病患者的外周血单个核细胞的分泌IL-2能力存在异常。
Objective To investigate the ability of peripheral blood mononuclear cells (PBMCs) to secrete IL-2 in patients with Parkinson’s disease. Methods The supernatant of PBMC cultured in vitro was detected by enzyme-linked immunosorbent assay (ELISA), and the content of IL-2 in the supernatant of PBMC stimulated by ConA was measured. Results The level of IL-2 secreted by unstimulated PBMC in patients with PD Ⅰ-Ⅲ was significantly higher than that in patients with PDⅡ-Ⅲ (P <0.05). After stimulated with ConA, the IL-2 production of PBMC from PDⅡ-Ⅲ patients was the lowest Compared with the normal control group, the IL-2 level in all the groups increased significantly after stimulation (P <0.05), but only in the healthy control group before and after stimulation (P <0.05). Conclusion Peripheral blood mononuclear cells from patients with Parkinson’s disease have abnormal secretion of IL-2.