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目的:研究扁担藤总黄酮和总三萜的提取工艺及抗氧化活性。方法:采用正交试验优化扁担藤中总黄酮和总三萜的最佳提取工艺,并采用FRAP法、水杨酸法和ABTS法测定其抗氧化能力。结果:扁担藤总黄酮提取的最佳条件为70%乙醇在70℃超声波提取3次,每次1 h;总三萜提取的最佳条件为60%乙醇在70℃微波提取2次,每次5 min。与对照品抗坏血酸和茶多酚相比较,富含黄酮的扁担藤的Fe3+还原抗氧化能力和清除ABTS+.能力均大于茶多酚,但清除OH.的能力均小于二者。结论:超声法提取总黄酮和微波法提取总三萜效果最好,扁担藤具有一定的抗氧化性能。
Objective: To study the extraction process and antioxidant activity of total flavonoids and total triterpenes in Psidium. Methods: The optimum extraction process of total flavonoids and total triterpenes from Plectranthus tomentosa was optimized by orthogonal test. The antioxidant activity of the extract was determined by FRAP, SA and ABTS methods. Results: The optimal extraction conditions of total flavonoids from Plectranthinovoltum were: 70% ethanol was extracted three times by ultrasonic wave at 70 ℃ for 1 h; the optimum conditions for total triterpene extraction were 60% ethanol extracted twice at 70 ℃, 5 min. Compared with the reference substance ascorbic acid and tea polyphenols, the flavonoids-rich ramification of Fe (superscript 3 +) reduced the antioxidant capacity and abscised ABTS +, but the capacity of removing OH was less than that of tea polyphenols. Conclusion: Ultrasonic extraction of total flavonoids and microwave extraction of the best total triterpene, Plum has some anti-oxidation properties.