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当硒的含量小于0．01ppm时，用荧光分光光度法测定硒，520nm处的检测峰将受到干扰．用二阶导教对光谱进行处理，可消除这些干扰．该方法的检测限达0．1ppb，回收率为98．8％－101％，变异系数为0.057．
When the content of selenium is less than 0.01 ppm, the detection peak at 520 nm will be disturbed when selenium is determined by fluorescence spectrophotometry. Using second-order teaching to process the spectrum eliminates these disturbances. The detection limit of the method is 0.1ppb, the recovery rate is 98.8% -101%, and the coefficient of variation is 0.057.