在小麦的花粉单倍体育种中,由于染色体自然加倍的频率很低,而往往使诱导获得的大量花粉植株最后很少能够结实,影响了育种效果。因此,对花粉植株进行有效的染色体加倍处理,成为小麦花粉单倍体育种重要环节之一。然而到目前为止,人工加倍处理的效率不仅低,而且很不稳定。几年来,我们曾采用分蘖基部穿线引入秋水仙碱溶液的方法进行处理。但加倍的效果很不理想;而 In the pollen haploid breeding of wheat, because of the natural frequency of chromosome doubling is very low, but often to induce a large number of pollen plants rarely able to end, affecting the breeding effect. Therefore, pollination of pollen plants effective chromosome doubling, wheat pollen haploid breeding an important part. However, so far, the efficiency of manual double processing is not only low but also very unstable. In recent years, we have adopted the method of introducing the colchicine solution into the basal part of the tiller. However, the effect of doubling is not ideal;