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利用公共的真菌基因组数据库资源,对核盘菌(Sclerotinia sclerotiorum)和灰葡萄孢(Botrytis cinerea)基因组中SSRs的结构类型、分布、丰度及最长序列等进行了系统分析,并与已经研究过的禾谷镰孢菌(Fusarium graminearum),稻瘟病菌(Magnaporthe grisea)和黑粉菌(Ustilago maydis)等几种植物病原真菌基因组中的SSRs进行了比较。结果表明:核盘菌和灰葡萄孢基因组中的SSRs非常丰富,分别为6539和8627个,并且在结构类型和分布规律上具有一定的相似性;与其他几种病原真菌相比,核盘菌和灰葡萄孢基因组中长重复的四、五、六核苷酸基序更为丰富,从而使得这两种真菌具有更高的变异性。同时,我们发现真菌基因组中SSRs的丰度与基因组的大小及GC含量没有必然的关系。文章对核盘菌和灰葡萄孢基因组中SSRs的丰度、出现频率及最长基序的分析为快速、便捷地设计多态性丰富的SSRs引物提供了有益的信息。
The structure, distribution, abundance and longest sequence of SSRs in the genomes of Sclerotinia sclerotiorum and Botrytis cinerea were systematically analyzed by using the public fungal genome database. Fusarium graminearum, Magnaporthe grisea and Ustilago maydis were compared in the genomes of several plant pathogenic fungi. The results showed that the SSRs of S. sclerotiorum and Botrytis cinerea were very rich, with 6539 and 8627, respectively, and had certain similarities in the structure types and distribution patterns. Compared with several other pathogenic fungi, And Botrytis cinerea genome longer repeat four, five, six nucleotide motifs more abundant, making the two fungi have a higher variability. At the same time, we found that the abundance of SSRs in the fungal genome is not necessarily related to the genome size and GC content. The analysis of the abundance, frequency of occurrence and longest motif of SSRs in S. sclerotiorum and Botrytis cinerea genomes provides useful information for the rapid and convenient design of polymorphism-rich SSR primers.