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目的探讨DPPⅣ基因高表达对卵巢癌细胞系恶性行为的影响及其机制。方法应用体外增殖能力、黏附和侵袭试验检验HO-8910PM、HOPcDNA和HODPP Ⅳ细胞恶性行为差异;利用流式细胞技术、吖啶橙染色、DNA ladder、透射电镜以及Western blot检测Fas/Bcl-2基因蛋白表达水平,探讨HO-8910PM、HOPcDNA和HODPP Ⅳ细胞中凋亡状况。结果 DPPⅣ基因高表达可显著抑制卵巢癌细胞的增殖、黏附和侵袭能力;同时HODPP Ⅳ细胞的凋亡率、Fas基因蛋白表达水平明显高于HOPcDNA和HO-8910PM细胞(P<0.05);HO-8910PM和HOPcDNA细胞的Bcl-2基因蛋白表达水平明显高于HODPP Ⅳ细胞(P<0.05)。结论 DPPⅣ基因高表达可抑制卵巢癌细胞的恶性行为;凋亡可能是DPPⅣ基因发挥生物学作用机制之一。
Objective To investigate the effect of DPPⅣ gene overexpression on the malignant behavior of ovarian cancer cell lines and its mechanism. Methods The malignant behaviors of HO-8910PM, HOPcDNA and HODPP IV cells were examined by in vitro proliferation, adhesion and invasion assays. Fas / Bcl-2 gene was detected by flow cytometry, acridine orange staining, DNA ladder, transmission electron microscopy and Western blot Protein levels in HO-8910PM, HOPcDNA and HODPP IV cells. Results The high expression of DPPⅣ gene significantly inhibited the proliferation, adhesion and invasion of ovarian cancer cells. Meanwhile, the apoptotic rate and the expression of Fas gene in HODPP Ⅳ cells were significantly higher than those in HOPcDNA and HO-8910PM cells (P <0.05) The protein expression of Bcl-2 in 8910PM and HOPcDNA cells was significantly higher than that in HODPP IV cells (P <0.05). Conclusions DPP ¢ ñ gene overexpression can inhibit the malignant behavior of ovarian cancer cells. Apoptosis may be one of the mechanisms by which DPP ¢ ö gene plays a biological role.