成年Sprague-Dawley大鼠在戊巴比妥钠麻醉下,于垂体后叶直视下注入CT-HRP和WGAHRP混合液0.5μl,12小时后,经侧脑室注入秋水仙素120μg。再存活12～24小时后,用3.5％多聚甲醛经升主动脉灌注固定。连续冰冻切片,厚40μm。TMB成色后,再用DAB-硫酸镍胺方法加强。用抗酪胺酸羟化酶抗体孵育48小时后,用ABC方法呈色。镜下见有3种不同的神经元:1.单纯紫蓝色颗粒的神经元;2.单纯棕黄色标记的神经元;3. Adult Sprague-Dawley rats were anesthetized with sodium pentobarbitol and 0.5 μl of CT-HRP and WGAHRP mixture was injected into the posterior pituitary. Twelve hours later, colchicine 120 μg was injected into the lateral ventricle. Re-survival of 12 to 24 hours later, with 3.5% paraformaldehyde perfusion by ascending aorta. Continuous frozen section, thickness 40μm. TMB color, then DAB-nickel sulfate amine method to strengthen. After 48 hours of incubation with anti-tyrosine hydroxylase antibody, color was visualized using the ABC method. There are 3 kinds of different kinds of neurons in the microscope: 1. The simple purple-blue neurons; 2. The simple yellow-brown neurons;