Kakade和Liener于1969年发表了用三硝基苯磺酸(TNBS)试剂测定有效赖氨酸的分析程序。近十年来,国内外用此法测定谷物中赖氨酸含量的报道较多。上海生化研究所1976年也报道了用此法测定小麦中赖氨酸的含量。但是,此法操作很复杂,要求条件较高。我们研究了用TNBS试剂,在偏碱性溶液里与水稻蛋白质上ε—氨基的定量反应,样品中蛋白质不必经过盐酸水解以及乙醚抽提杂质等操作手续,而采取直接测定赖氨酸含量的方法。本法与氨基酸 Kakade and Liener published analytical procedures for the determination of effective lysine with trinitrobenzene sulfonic acid (TNBS) reagent in 1969. In the past ten years, there have been many reports of lysine content in cereals at home and abroad. Shanghai Institute of Biochemistry in 1976 also reported the use of this method to determine the content of lysine in wheat. However, this method is complicated to operate and requires high conditions. We studied the direct determination of lysine content by using TNBS reagent, quantitative reaction of ε-amino group with rice protein in alkaline solution and the procedure that protein in sample did not have to undergo hydrochloric acid hydrolysis and ether extraction of impurities. . This Law and Amino Acids