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目的通过体外实验探讨干酪乳酸菌N1115对人结肠癌细胞的增殖活性的影响。方法于2014年4月至2015年4月进行相关研究,通过人结肠癌上皮细胞的培养,确定最佳细胞铺板密度和培养时间;干酪乳酸菌N1115的培养,配置不同浓度的灭活菌悬液及细菌培养上清液;用MTT法研究不同浓度灭活菌液、细菌发酵液及细菌与巨噬细胞J774.1共同培养液作用12、24、48h对人结肠癌上皮细胞增殖的抑制作用。结果发酵液除12与28h的5%配比时与对照组无差异,其余各组与对照组的差异均有统计学意义(P<0.01),且对细菌增殖抑制率均大于0.86,各实验组间差异无统计学意义(P>0.05);共培液5%和10%配比培养24h时与对照组差异有统计学意义(P<0.01),对细菌增殖抑制率约为0.2,其余各组均与对照组差异无统计学意义(P>0.05),各实验组间差异无统计学意义(P<0.01);灭活菌液与对照组差异均无统计学意义(P>0.05),组间差异无统计学意义(P>0.05)。结论干酪乳酸菌N1115发酵液对人结肠癌细胞的增殖有显著抑制作用,灭活菌悬液及J细胞共培液对人结肠癌细胞增殖抑制作用不明显。
Objective To investigate the effect of Lactobacillus casei N1115 on the proliferation of human colon cancer cells in vitro. Methods From April 2014 to April 2015, related studies were conducted to determine the optimal plating density and culture time of human colon carcinoma epithelial cells. The culture of Lactobacillus casei N1115 was performed with different concentrations of inactivated bacterial suspension and Bacterial culture supernatant; MTT assay with different concentrations of inactivated bacterial liquid, bacterial fermentation broth and bacteria and macrophage J774.1 co-culture solution of 12,24,48 h on human colon cancer cell proliferation inhibition. Results There was no significant difference between the control group and the other groups except for 5% of 12 and 28 h (P <0.01), and the inhibitory rates of bacterial growth were all higher than 0.86 There was no significant difference between the two groups (P> 0.05). The difference of 5% and 10% co-culture medium with control group was significant (P <0.01), and the inhibition rate to bacterial proliferation was about 0.2 There was no significant difference between each group and the control group (P> 0.05), and there was no significant difference between the experimental groups (P <0.01). There was no significant difference between the inactivated bacteria liquid and the control group (P> 0.05) , There was no significant difference between groups (P> 0.05). Conclusion Lactobacillus casei N1115 fermentation broth significantly inhibited the proliferation of human colon cancer cells. Inactivated bacterial suspension and J cell co-culture had no obvious inhibitory effect on the proliferation of human colon cancer cells.