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目的通过3种分光光度法之间差异的比较和适用性考量,筛选出适合检测蜂胶中总黄酮含量的分光光度法。方法采用同批次样品,对蜂胶(国产蜂胶和巴西绿蜂胶)中总黄酮检测方法进行回收率和含量测定,并对结果进行分析。结果巴西绿蜂胶粉3种方法的检测结果为4.99~9.34 g/100 g;国产蜂胶粉3种方法的检测结果为7.82~17.97 g/100 g,其RSD为24.2%~31.2%;可见3种方法测定结果偏差较大。结论 GB/T20574-2006的方法最适合蜂胶中总黄酮的检测。
OBJECTIVE To screen the spectrophotometric method suitable for the determination of total flavonoids in propolis by comparing the differences between three spectrophotometries and applicability considerations. Methods The same batch of samples was used to determine the recovery and content of total flavonoids in propolis (domestic propolis and Brazil green propolis), and the results were analyzed. Results The detection results of three methods of Brazilian green propolis powder were 4.99-9.34 g / 100 g. The results of three methods of domestic propolis powder were 7.82-17.97 g / 100 g with RSD of 24.2% -31.2% Method measurement results deviate large. Conclusion GB / T20574-2006 method is most suitable for the detection of total flavonoids in propolis.