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目的 对四川省社鼠分离株SN7进行生物学和分子生物学鉴定。方法 应用单克隆抗体抗原性分析和空斑减少中和试验 (PRNT)和PCR分型方法分析、克隆测定了SN7株的M和S片段基因 ,并同汉坦病毒其他病毒株进行比较。结果 单克隆抗体和PRNT、PCR分型方法均无法对SN7株定性 ,序列分析表明SN7毒株与现有的HTN型毒株M片段同源性为 80 .2 %～ 87.1% ,差异高达 12 .9%～ 19.8% ;S片段为 76 .6 %～ 92 .0 % ,差异也高达 8%～ 2 3.4 % ,而与SEO毒株M片段的同源性为 70 .0 %～ 71.6 % ,S片段为 71.0 %～ 72 .2 % ,SN7株病毒可以定型为HTN型病毒。结论 SN7株为HTN型的新亚型毒株。
Objective To identify the biological and molecular biological features of SN7 isolated from Sichuan Province. Methods The monoclonal antibody antigenicity analysis and plaque reduction neutralization test (PRNT) and PCR typing were performed. The M and S gene of SN7 strain was cloned and compared with other Hantavirus strains. Results The results showed that the SN7 strain could not be identified by the monoclonal antibody, PRNT and PCR. The sequence analysis showed that the SN7 strain shared 80.2% -87.1% identity with the existing HTN strain M fragment with a difference of 12. 9% -19.8%; S fragment was 76.6% -92.0%, the difference was as high as 8% -2.34%, while it was 70.0% -71.6% with M segment of SEO strain, S The fragment ranged from 71.0% to 72.2%. The SN7 strain could be screened as HTN-type virus. Conclusion The SN7 strain is a new subtype of HTN strain.