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目的:比较心肺复苏动物模型的2种心脏骤停判断标准,以确立适合于心脏骤停后综合征研究的心脏骤停判断标准。方法:大耳白兔30只,随机分为心电图标准组(A组)、动脉压标准组(B组)。采用夹闭气管建立心脏骤停模型,分别使用2种不同的心脏骤停判断标准,心脏骤停5min时立即复苏,观察复苏成功率和兔存活时间,监测心脏骤停前、复苏后24、48、72、96、120h6个时间点兔血神经元特异性烯醇化酶(NSE)、谷丙转氨酶(ALT)、血肌酐及肌钙蛋白I水平,分析复苏后多器官功能障碍综合征(MODS)发生情况。结果:心肺复苏即刻成功率A组为33%,B组为86%,2组比较有显著差异(P<0.05)。兔存活时间A组为(1.38±1.04)h,B组存活时间为(58.0±41.5)h,2组比较有显著差异(P<0.01)。B组兔复苏后24、48、72h时间点血NSE、ALT、肌酐及肌钙蛋白I水平明显升高,与心脏骤停前比较有显著差异(P<0.01)。复苏后24～72h,兔出现多器官功能障碍综合征(MODS)表现。结论:以平均动脉压为标准判断心脏骤停优于心电图标准,适用于标准化心脏骤停后综合征的兔模型的建立。
OBJECTIVE: To compare two criteria for cardiac arrest in an animal model of cardiopulmonary resuscitation to establish criteria for determining cardiac arrest suitable for post-cardiac arrest syndrome studies. Methods: Thirty rabbits were randomly divided into group A (standard electrocardiogram) and group B (standard arterial pressure). Cardiac arrest model was established by clogged trachea. Two different criteria of cardiac arrest were used respectively. Immediate resuscitation was performed 5 minutes after cardiac arrest. The success rate of resuscitation and the survival time of rabbits were observed. Before cardiac arrest, 24,48 after resuscitation (NSE), alanine aminotransferase (ALT), serum creatinine and troponin I were measured at 72, 96 and 120h after 6 days of resuscitation, and the changes of multiple organ dysfunction syndrome (MODS) What happened? Results: Immediate success rate of cardiopulmonary resuscitation was 33% in group A and 86% in group B, with significant difference between the two groups (P <0.05). The survival time of rabbits was (1.38 ± 1.04) h in group A, and (58.0 ± 41.5) h in group B, with significant difference between the two groups (P <0.01). The levels of serum NSE, ALT, creatinine and troponin I in group B at 24, 48 and 72h after resuscitation were significantly higher than those before cardiac arrest (P <0.01). 24 ~ 72h after recovery, rabbits showed multiple organ dysfunction syndrome (MODS) performance. CONCLUSION: Cardiac arrest is superior to ECG criteria based on mean arterial pressure and is suitable for the establishment of a rabbit model of standardized post-cardiac arrest syndrome.