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目的探讨大鼠心肌梗死(MI)后不同时间,梗死组织基质细胞衍生因子-1(SDF-1)的表达以及与骨髓间充质干细胞(MSC)迁移的关系,为细胞移植提供可靠依据。方法在MI后不同时间,用PCR测定梗死组织中SDF-1表达的水平。提取梗死心肌组织提取液,在Costar Transwell双层细胞培养皿观察MI后不同时间梗死心肌组织对MSC迁移能力的影响。结果SDF-1在组织脏器内的表达存在差异,MI梗死心肌组织中SDF-1的表达呈现出时间变化的趋势,梗死后48h达到高峰水平。梗死后的心肌组织对MSC迁移的影响与SDF-1的表达呈相同的变化程序,梗死后48h对MSC的趋化作用最强。经SDF-1的受体CXCR4特异性阻滞剂处理后,MSC向梗死组织提取液迁移的能力受到明显抑制。结论MI后的早期,局部组织中SDF-1的表达明显升高,并可在MI后2周内维持在一个相对高的水平,其对于移植细胞向梗死区域迁移具有重要的作用。
Objective To investigate the expression of stromal cell-derived factor-1 (SDF-1) and its relationship with the migration of bone marrow mesenchymal stem cells (MSCs) at different time points after myocardial infarction (MI) in rats and provide a reliable basis for cell transplantation. Methods The levels of SDF-1 in infarcted tissues were detected by PCR at different time points after MI. Myocardial tissue extracts from infarcted myocardium were collected and cultured in Costar Transwell double-layer cell culture dishes to observe the effects of myocardial infarction on migration of MSC at different time points after myocardial infarction. Results The expression of SDF-1 in tissues and organs was different. The expression of SDF-1 in infarcted myocardium of MI showed a time-varying trend, reaching the peak 48h after infarction. The effect of myocardial infarction on MSC migration was the same as that of SDF-1 expression, and the strongest chemotaxis was observed at 48h after infarction. After SDF-1 receptor CXCR4-specific blocker treatment, MSCs migration to the infarct tissue extract was significantly inhibited. Conclusion The expression of SDF-1 in local tissues is obviously increased in the early stage after MI and can be maintained at a relatively high level within 2 weeks after MI, which plays an important role in the migration of transplanted cells into the infarct area.