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目的建立一种肉中志贺氏菌的快速检测方法。方法利用FTA(Flinders Technology Associates)滤膜从肉中提取志贺氏菌基因组DNA,以志贺氏菌属的ipaH基因为模板设计引物进行PCR反应得到369 bp片段,经测序证实该片段为目的扩增产物。结果采用建立的FTA滤膜结合PCR检测肉中志贺氏菌的方法,人工污染牛肉、羊肉、猪肉、鸡肉匀浆液的检出限均为8×101CFU/ml匀浆,6 h即可完成整个检测过程。结论该方法灵敏度高、耗时短,为预防志贺氏菌食物中毒、控制细菌性痢疾的暴发流行提供了新的检测方法。
Objective To establish a rapid detection method of Shigella in meat. Methods The genomic DNA of Shigella was extracted from meat by FTA (Flinders Technology Associates) filter. The ipaH gene of Shigella was used as a template to design a PCR primer to obtain a 369 bp fragment. The fragment was confirmed by sequencing Increase products. Results The established FTA filter combined with PCR method for the detection of Shigella in meat, the detection limits of artificially contaminated beef, mutton, pork and chicken homogenate were all 8 × 101CFU / ml homogenate, 6 h to complete the whole Testing process. Conclusion The method is sensitive, time-consuming and provides a new detection method for preventing Shigella food poisoning and controlling the outbreak of bacillary dysentery.