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本文采用16～18d胎龄的大鼠皮层细胞进行分离培养，以培养上清液中乳酸脱氢酶（LDH）活性作为细胞损伤的指标，分别观察了缺氧和过量谷氨酸对皮层神经元的影响以及右美沙芬的保护作用。实验结果表明，外源性谷氨酸（10和50μmol／L）和缺氧（5h）均引起LDH释放增加，而这种作用皆被右美沙芬所抑制。提示右美沙芬对此有明显的保护作用。以上结果说明谷氨酸兴奋毒性与NMDA受体在缺血性脑损伤过程中起着重要的作用。
In this paper, 16 ~ 18d gestational age rat cortical cells were isolated and cultured to culture supernatant lactate dehydrogenase (LDH) activity as an indicator of cell damage were observed hypoxic and excessive glutamate on cortical neurons And the protective effect of dextromethorphan. The results showed that both exogenous glutamate (10 and 50μmol / L) and hypoxia (5h) caused an increase of LDH release, which was inhibited by dextromethorphan. Tips dextromethorphan obvious protective effect. The above results indicate that glutamate excitotoxicity plays an important role in the process of ischemic brain injury.