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目的:探讨薏苡仁多糖防治化学性肝损伤的作用。方法:分别以0.33g/KgBW、0.67g/KgBW、2.00g/KgBW(低、中、高)3个剂量组对小鼠每日经口进行灌胃,空白对照组和模型对照组给予蒸馏水灌胃。30d后将各组动物禁食16h,模型组及各剂量组动物按0.01mL/gBW剂量一次灌胃给予CCl4染毒,各受试组染毒4h后继续给予受试样品。染毒24h后摘取眼球取血,分离血清,测定ALT、AST,同时处死动物,取肝脏进行组织病理学检查。结果:薏苡仁多糖对小鼠的体质量增长无影响;CCl4模型对照组小鼠的ALT、AST含量比空白对照组高(P<0.05),低、中剂量组的ALT、AST值与CCl4模型对照组比较明显降低,差异有统计学意义(P<0.05);肝脏组织病理学检查结果表明薏苡仁多糖有保肝作用。结论:薏苡仁多糖具有防治CCl4化学性肝损伤的作用。
Objective: To investigate the effect of Coixan polysaccharide on prevention and treatment of chemical liver injury. Methods: Mice were intragastrically orally administered with 3 doses of 0.33g / KgBW, 0.67g / KgBW and 2.00g / KgBW (low, medium and high) respectively. The blank control group and model control group were given distilled water stomach. After 30 days, the animals in each group were fasted for 16 hours. CCl4 was given intragastrically to the model group and each dose group at a dose of 0.01 mL / gBW. The test groups were given the test sample after 4 hours of exposure. After exposure for 24 hours, the eyeballs were taken for blood collection, serum was separated, ALT and AST were determined, and animals were sacrificed at the same time. The liver was taken for histopathological examination. Results: The Coixan polysaccharide had no effect on the growth of body weight in mice. The levels of ALT and AST in the CCl4 model control group were higher than those in the blank control group (P <0.05) The control group was significantly lower, the difference was statistically significant (P <0.05); liver histopathological examination results show that Coixan polysaccharides have hepatoprotective effect. Conclusion: Coixan polysaccharide has the effect of preventing and curing CCl4 chemical liver injury.