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为了解Ⅵ型胶原蛋白在人卵巢卵泡的生长过程中的表达,用鼠抗人Ⅵ型胶原蛋白特异性单克隆抗体,做免疫组化测定Ⅵ型胶原蛋白在卵泡生长过程中的分布特点,用原位杂交法检测卵泡组织中Ⅵ型胶原蛋白的mRNA表达。 人卵巢组织(包括卵泡)取自月经规则因子宫颈癌需做子宫附件切除的病人,年龄39～46岁,均未接受激素治疗,术前也无卵巢功能或内分泌紊乱。取材在月经周期中的具体时间判定根据月经史及基础体温测定。周期为28～30 d。卵泡期分为早卵泡期(周期第1～5 d)4例;中卵泡期(周期第6～9 d)3例;排卵前期(周期第10～14 d)5例。卵巢组织中未发现癌转移灶。 结果:免疫组化染色发现,取自早卵泡期的原始卵泡和初级卵泡未呈现Ⅵ型胶原蛋白阳性染色。中卵泡期次级卵
To understand the expression of collagen type Ⅵ in the growth of human ovarian follicles, the distribution characteristics of type Ⅵ collagen during follicular growth were determined by immunohistochemistry using mouse anti-human Ⅵ-type collagen-specific monoclonal antibody. In Situ Hybridization Assay for mRNA Expression of Collagen VI in Follicular Tissues. Human ovarian tissue (including follicles) taken from the regular menstrual cervical cancer due to uterine resection of patients, aged 39 to 46 years old, did not receive hormone therapy, ovarian function or endocrine disorders before surgery. Draw in the menstrual cycle in the specific time to determine menstrual history and basal body temperature determination. The period is 28 ~ 30 d. Follicular phase was divided into early follicular phase (cycle 1 ~ 5 d) 4 cases; follicular phase (cycle 6 ~ 9 d) in 3 cases; ovulation (cycle 10 ~ 14 d) in 5 cases. No ovarian cancer metastases were found. Results: Immunohistochemical staining showed that the primordial follicles from the early follicular phase and the primary follicles did not show type Ⅵ collagen-positive staining. Middle follicular stage secondary eggs