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目的探讨缺氧缺血性脑损伤(HIBD)后脑内神经突起外生及突触重建标志蛋白——生长相关蛋白-43(GAP-43)mRNA表达的变化及碱性成纤维细胞生长因子(bFGF)对其表达的影响。方法取20只正常Wis-tar大鼠为正常对照组,另取54只7日龄大鼠随机分为假手术组、HIBD组和bFGF治疗组。HIBD模型参照Rice法制成。治疗组于损伤后腹腔注射bFGF、每天4U/g,直至处死;HIBD组腹腔注射等量生理盐水。采用逆转录-聚合酶链式反应(RT-PCR)技术测定3组大鼠损伤后1 d,3 d,7 d(即8,10,14日龄)以及正常大鼠2 d,5 d,7 d及14 d脑内GAP-43 mRNA的表达。结果正常大鼠生后2天GAP-43 mRNA表达较低(0.16±0.19),以后逐渐增强,7d高峰表达,至14 d仍有较高水平表达(1.0±0.70)。HIBD后1 d双侧脑组织GAP-43 mRNA表达较假手术组明显减低。bFGF治疗3 d后,与同组治疗后1天及同日龄HIBD组比较,双侧脑组织GAP-43 mRNA表达显著增加,至7 d仍维持较高表达。结论新生大鼠生后脑内GAP-43mRNA表达呈时间依赖性变化,与脑发育及功能成熟过程密切相关,bFGF对脑损伤后细胞增殖及功能重建有一定促进作用。
Objective To investigate the changes of neurite outgrowth and synaptic remodeling marker protein-GAP-43 (GAP-43) mRNA expression in brain after hypoxic-ischemic brain damage (HIBD) and the effect of basic fibroblast growth factor ) On its expression. Methods Twenty normal Wis-tar rats were selected as normal control group. Another 54 7-day-old rats were randomly divided into sham-operation group, HIBD group and bFGF-treated group. HIBD model made with reference to Rice law. In the treatment group, bFGF was intraperitoneally injected after injury, 4U / g daily until sacrifice; HIBD group was injected intraperitoneally with normal saline. The damage of rats in 1, 3, 7 days (8, 10, 14 days old) and normal rats 2 d, 5 d, 5 d after injury were detected by reverse transcription polymerase chain reaction (RT- The expression of GAP-43 mRNA on the 7th and 14th day in the brain. Results The expression of GAP-43 mRNA in normal rats was lower (0.16 ± 0.19) 2 days after birth, then gradually increased and peaked at 7 days, and remained high at 14 days (1.0 ± 0.70). One day after HIBD, the expression of GAP-43 mRNA in bilateral brain tissues was significantly lower than that in sham operation group. After 3 days of bFGF treatment, the expression of GAP-43 mRNA in bilateral brain tissues was significantly increased compared with that of the same group at 1 day and the same-day HIBD group, and remained high after 7 days of bFGF treatment. Conclusions The expression of GAP-43mRNA in neonatal rats after birth is closely related to the process of brain development and function maturation, and bFGF may promote the proliferation and functional remodeling after brain injury.