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目的研究活动期多发性肌炎患者外周血白细胞细胞因子信号转导蛋白抑制因子(SOCS)1、SOCS2、SOCS3和细胞因子诱导的含SH2区域蛋白1(CIS)与正常人表达的差异,探讨SOCS在多发性肌炎发病中可能的作用。方法 2011年6月-12月,采用实时荧光定量聚合酶链反应法检测了14例活动期多发性肌炎患者和14例正常人外周血白细胞中SOCS1、SOCS2、SOCS3和CIS1基因的相对表达量。结果与对照组相比,多发性肌炎症患者外周血白细胞基因SOCS 1～3表达明显降低(P值均<0.05),CIS1基因的表达较对照组明显升高(P<0.05),差异有统计学意义。结论 SOCS基因家族可能参与了多发性肌炎的发病,该蛋白分子家族的成员可能会成为多发性肌炎治疗的一种新的候选基因。
Objective To investigate the differences of peripheral blood leukocyte cytokine signaling inhibitor 1 (SOCS) 1, SOCS2, SOCS3 and cytokine-induced SH2-containing domain 1 (CIS) expression in active stage patients with polymyositis and normal subjects, Possible role in the pathogenesis of polymyositis. Methods The relative expression levels of SOCS1, SOCS2, SOCS3 and CIS1 in peripheral blood leukocytes from 14 patients with active myositis and 14 normal controls were detected by real-time fluorescence quantitative polymerase chain reaction from June to December in 2011. . Results Compared with the control group, the expression of SOCS 1 ~ 3 in peripheral blood leukocyte was significantly lower in patients with polymyositis (P <0.05), and the expression of CIS1 was significantly higher than that in the control group (P <0.05) Significance of learning. Conclusion SOCS gene family may be involved in the pathogenesis of polymyositis. Members of this protein family may become a new candidate gene for the treatment of polymyositis.