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对4个国家(地区)的13份西番莲种质用ITS、trnL-F、trnH-psbA等3种DNA条形码进行遗传多样性分析。结果表明,trnH-psbA条形码扩增出来的DNA序列的GC含量最低(27%),ITS条形码的DNA序列GC含量最高(63%);而trnL-F条形码扩增的DNA序列GC含量在不同种质间差异最大,在52.6%~62.3%之间。将3种DNA条形码序列在GeneBank中进行Blast比对,ITS和trnL-F序列基本与已登录种序列一致,达到77%以上,而trnH-psbA条形码序列与已登录种不一致。ITS条形码在序列间基因进化多样性分析显示,两两序列之间都具有差异;trnLF条形码序列的两两比较发现T1,T10,T11,T12,T13条形码序列之间无差异;trnH-psbA条形码序列的两两比较分析的差异最小,而H4与其他的序列差异较大,在13.3以上。从系统进化树来看,trnH-psbA条形码序列除了H4之外,其他样品聚为一类;trnL-F条形码序列中有5个不存在遗传差异;ITS条形码序列聚类的结果显示,每种西番莲均存在一定的遗传距离,这与基因多样化分析的结果一致。表明ITS在不同种质之间具有较高的多样性,可考虑应用于种质鉴定。
Thirteen passiflora germplasms in four countries (regions) were analyzed for genetic diversity using three DNA barcodes, ITS, trnL-F, trnH-psbA. The results showed that the GC content of the trnH-psbA barcode was the lowest (27%), and the DNA content of the ITS barcode was the highest (63%). However, the GC content of the trnL-F barcode was different among different species Difference between the largest, between 52.6% ~ 62.3%. Blast comparison of the three DNA barcoding sequences in GeneBank showed that the sequence of ITS and trnL-F was basically consistent with the registered sequence, reaching more than 77%, while the trnH-psbA barcode sequence was inconsistent with the registered sequence. ITS barcode analysis of gene diversity between sequences showed that there was a difference between every two sequences; trnLF bar code sequence pairs of comparison found no difference between T1, T10, T11, T12, T13 barcode sequences; trnH-psbA barcode sequence The difference between pairwise comparisons was the smallest, while H4 was quite different from other sequences, above 13.3. In phylogenetic tree, trnH-psbA barcode sequence was clustered except for H4, and there was no genetic difference in trnL-F barcode sequence. The clustering results of ITS barcode sequence showed that each kind of western There is a certain genetic distance between Paphiopedilum and Pseudostellariae, which is consistent with the result of gene diversity analysis. It indicates that ITS has a high diversity among different germplasms and may be considered for germplasm identification.