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利用SRAP(Sequence-related amp lified polymorph ism)标记对来自国内外的45份鸭茅种质资源进行遗传多样性研究。21对引物扩增出438个条带,多态性条带为363条,多态性条带比率为82.08%,每对引物组合的多态性带数平均为17.29条。GS值范围在0.6248～0.9686间,平均GS值为0.7958,显示来源广泛的鸭茅种质资源间存在着丰富的遗传变异。聚类分析及主成分分析能将所有材料聚为4类,能较准确的反映材料的来源分布情况及供试材料的染色体倍性差异,表明鸭茅的遗传多样性与染色体倍性及地理分布密切相关。同时清楚地揭示出国产鸭茅品种遗传基础较为狭窄。本研究为育种和探讨鸭茅种质资源遗传变异奠定了较好的理论基础。
The genetic diversity of 45 accessions of Dactylis glomerulosum germplasm resources from China and abroad were studied by using SRAP (Sequence-related amputated polymorphism) markers. 21 pairs of primers amplified 438 bands, the number of polymorphic bands was 363, the percentage of polymorphic bands was 82.08%, and the average number of polymorphic bands per pair of primers was 17.29. The GS value ranged from 0.6248 to 0.9686 with the average GS value of 0.7958, indicating that there is abundant genetic variation among the germplasm resources of the widely sourced C. sinensis. Cluster analysis and principal component analysis can cluster all materials into four categories, which can more accurately reflect the source distribution of materials and chromosome ploidy differences of the tested materials, indicating that the genetic diversity and chromosome ploidy and geographical distribution closely related. At the same time it clearly reveals that the genetic basis of domestic Dactylogyrus breed is relatively narrow. This study laid a good theoretical basis for breeding and exploring the genetic variation of the germplasm resources of Dactylis glomerata.