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背景与目的:研究表明,内皮抑素能抑制肿瘤血管的生成,进而抑制肿瘤的生长和转移,然而有关内皮抑素治疗的研究报道却很少。本研究初步探讨了重组腺相关病毒介导内皮抑素对肿瘤生长和转移的作用。方法:用RT-PCR方法获得人内皮抑素基因,构建内皮抑素可分泌表达的通用型重组腺相关病毒(rAAV)载体,包装获得重组腺相关病毒rAAV-SS-Endostatin。以动物实验分析其抗肿瘤作用。结果:C57BL/6小鼠肌肉注射1011TUrAAC-SS-Endostatin一次,该重组病毒对小鼠黑色素瘤B16F10移植瘤生长的抑制率为57.1%,在实验肺转移模型中,对转移灶的抑制率为70.7%。结论:重组腺相关病毒介导的内皮抑素能有效抑制肿瘤的生长和转移。
BACKGROUND & OBJECTIVE: Studies have shown that endostatin can inhibit the formation of tumor blood vessels, thereby inhibiting tumor growth and metastasis, however, there are few reports on the treatment of endostatin. This study initially explored the effect of recombinant adeno-associated virus-mediated endostatin on tumor growth and metastasis. Methods: The human endostatin gene was obtained by RT-PCR and the recombinant adeno-associated virus (rAAV) vector secreting endostatin was constructed. The recombinant adeno-associated virus rAAV-SS-Endostatin was packaged and packaged. Animal experiments to analyze its anti-tumor effect. Results: The intramuscular injection of 1011TUrAAC-SS-Endostatin in C57BL / 6 mice resulted in an inhibition rate of 57.1% on the growth of mouse melanoma B16F10 xenografts. In the experimental lung metastasis model, the inhibition rate on metastasis was 70.7 %. Conclusion: Recombinant adeno-associated virus-mediated endostatin can effectively inhibit tumor growth and metastasis.