论文部分内容阅读
目的 建立放射性肠上皮损伤小鼠模型,观察肠上皮损伤病理变化规律.方法 分别以10,12,14,16和18 Gy剂量单纯照射小鼠腹部,筛选最小致死剂量.进而采用18 Gy剂量照射Lgr5-EGFP-ires-CreERT2转基因小鼠,每日观察小鼠体重,并在辐照后0.5、3.5和6d取小鼠小肠,观察小肠的病理学改变规律,如小肠绒毛长度、隐窝数目和G蛋白耦联受体5(LGR5)+肠道干细胞的数量变化.结果 18 Gy为单纯腹部照射建立放射性肠上皮损伤小鼠模型的最小致死剂量;与未处理组相比,腹部照射后小鼠体重逐步减轻、小肠绒毛变短、隐窝数目减少、LGR5+肠隐窝干细胞数量在短时间内迅速减少.结论 实验采用18 Gy剂量单纯辐照小鼠腹部,建立了放射性肠上皮损伤小鼠模型,初步探讨了放射性肠上皮损伤病理规律,为急性放射损伤救治的研究提供了良好的研究模型.“,”Objective To establish a mouse model of intestinal epithelium irradiation damage and observe its pathological changes. Methods First, the minimum lethaldose of irradiation was chosen. Mice were divided depending on the abdominal irradiation dosage of 10, 12, 14, 16 and 18 Gy. Lgr5-EGFP-ires-CreERT2 mice were abdominal irradiation at the dose of 18 Gy, the weight of mice were recorded everyday, intestinal tissues were scarified at 0.5, 3.5, 6 day to investigate pathological changes such as length of intestinal villi, number of crypt and the change of the LGR5+ intestinal stem cells. Results 18 Gy as the minimum lethal dose for abdominal irradiation in intestinal epithelium irradiation damage mouse model in this test. Compared with the untreated group, the weight loss, villus short, crypt number reduction and the number of LGR5+ intestinal stem cells rapidly deceased in a short term. Conclusion This study establishes a mouse model of intestinal epithelium irradiation damage with abdominal irradiation at the dose of 18 Gy, observes the pathological changes of irradiation induced intestinal epithelium damage and provides a model for treating the acute radiation sickness.