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目的:研究化积益肝煎对小鼠肝癌腹水瘤细胞系H22治疗的作用机制。方法:小鼠右腋下皮下种瘤造成实体瘤模型,将24只小鼠分为三组,生理盐水组、化积益肝煎组(中药组)和5-氟脲嘧啶组(西药组)各8只,用药14天后取瘤,称重计算抑瘤率,采用免疫组化技术检测C–erb B-2及Fasl的表达。结果:中药组、西药组大鼠瘤重显著低于对照组(P<0.05);中药组、西药组的抑瘤率分别为32.11%、38.61%;C–erb B-2及Fasl在三组中表达结果经秩和检验,与对照组比较,中药组C–erb B-2、Fasl表达减弱,差异均有统计学意义(P<0.05),而与西药组比较差异无统计学意义(P>0.05)。结论:化积益肝煎对肝癌H22细胞的增殖有明显的抑制作用,其作用机制与C–erb B-2、Fasl表达有关。
Objective: To study the mechanism of Huiji Yigan Decoction on the treatment of mouse hepatocellular carcinoma cell line H22. Methods: The right subaxillary subcutaneous tumor of mice caused the solid tumor model. The 24 mice were divided into three groups: normal saline group, HGYGG (Chinese medicine group) and 5-fluorouracil group (Western medicine group) Twenty-eight rabbits were sacrificed after 14 days. The tumor inhibition rate was calculated by weighing and the expression of C-erb B-2 and Fasl was detected by immunohistochemistry. Results: The tumor weight of Chinese medicine group and Western medicine group was significantly lower than that of the control group (P <0.05). The tumor inhibition rates of Chinese medicine group and western medicine group were 32.11% and 38.61% respectively. C-erb B-2 and Fasl in three groups (P <0.05). However, the expression of C-erb B-2 and Fasl in traditional Chinese medicine group was significantly lower than that in the control group (P <0.05), while the difference was not statistically significant > 0.05). Conclusion: HGYG can obviously inhibit the proliferation of hepatocellular carcinoma H22 cells, and its mechanism is related to the expression of C-erb B-2 and Fasl.