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目的探讨胆固醇结石的成因。方法用Holan方法测定胆汁的成核时间,胆汁脂质测定分别用三氯化铁显色法、硫酸—过氯酸消化法、磷钼酸比色法,胆固醇饱和度计算由Careg表查出,胆汁总蛋白和载脂蛋白A-I分别用考马斯亮蓝蛋白法和免疫透射法检测。结果胆固醇结石成核时间较胆色素结石和外胆囊结石明显缩短,胆固醇结石成核时间与胆固醇饱和度无相关性,成核时间缩短患者胆汁中总蛋白浓度明显增加,而载脂蛋白A-I浓度降低。结论胆固醇结石成核时间明显缩短,成核时间长短与胆固醇饱和度无关,胆汁总蛋白浓度增加可促进胆固醇结石的形成,而载脂蛋白A-I可抑制胆固醇结石的形成。
Objective To investigate the causes of cholesterol gallstone. Methods The bile nucleation time was determined by the method of Holan. The bile lipids were detected by the Careg table using ferric chloride colorimetric method, sulfuric acid - perchloric acid digestion method, phosphomolybdic acid colorimetric method and cholesterol saturation calculation respectively. Total bile protein and apolipoprotein A-I were detected by Coomassie blue staining and immuno-transmission assay. Results Cholesterol stone nucleation time was significantly shorter than that of bile pigment stones and external gallbladder stones. Cholesterol stone nucleation time was not correlated with cholesterol saturation. The total protein concentration in bile was significantly increased in patients with shortened nucleation time. However, apolipoprotein A-I Decreased concentration. Conclusion Cholesterol stone nucleation time was significantly shortened, the length of nucleation time and cholesterol saturation has nothing to do, total bile protein concentration can promote the formation of cholesterol stones, and apolipoprotein A-I can inhibit the formation of cholesterol stones.