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本研究以青海省马铃薯品种“青薯9号”无菌苗为材料,利用10%PEG-6000溶液模拟干旱胁迫,运用双向电泳技术,对干旱胁迫后的马铃薯茎段蛋白进行差异蛋白组学分析。结果表明:无菌苗在10%PEG-6000溶液模拟干旱胁迫下,其茎段蛋白表达发生了显著变化:其中共有139个蛋白点得到匹配,检测出127个差异表达的蛋白点,108个表达量表现为显著上调,12个显著下调,得到7个新增蛋白。这表明,在干旱胁迫环境条件下,马铃薯为了响应水分亏缺可能通过调控相应的代谢路径和水平以提高自身对胁迫环境的适应性。
In this study, the sterile potato seedlings “Qingshu 9” in Qinghai Province were used as materials to simulate drought stress with 10% PEG-6000 solution. Two-dimensional electrophoresis technique was used to detect the differential proteins of stem segments of potato after drought stress Analysis. The results showed that under the drought stress of 10% PEG-6000 solution, the aseptic seedlings had a significant change in stem protein expression: a total of 139 protein spots were matched, 127 differentially expressed protein spots were detected and 108 were expressed The amount showed a significant up-regulation, 12 significant down-regulation, resulting in seven new proteins. This indicates that under drought stress conditions, in order to respond to water deficit, potato may regulate its corresponding metabolic pathways and levels to improve its adaptability to stress environment.