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在实验条件下确立了贯叶连翘中金丝桃素超临界CO2萃取的最佳工艺条件。在单因素实验基础上,采用响应面分析法,对影响超临界萃取物中金丝桃素含量的主要因素包括萃取温度、萃取压力与夹带剂浓度(乙醇体积分数)进行优化,建立了影响因素与响应值(金丝桃素含量)之间的函数关系。根据回归方程寻优得出最佳工艺条件。最佳工艺条件为以体积分数78.6%的乙醇作动态夹带剂,萃取温度50.3℃、萃取压力25.29 MPa,在此条件下,设定乙醇流速0.5 mL.min-1、CO2流速2 L.min-1,萃取2 h,超临界萃取物中金丝桃素含量平均达到1.102%,大大高于传统提取方法(碱醇溶液提取),适用于贯叶连翘中金丝桃素的提取。
Under the experimental conditions, the optimal conditions for the extraction of hypericin from Hypericum perforatum L. by supercritical CO2 were established. Based on the single factor experiment, response surface analysis was used to optimize the main factors influencing the content of hypericin in the supercritical fluid extraction, including extraction temperature, extraction pressure and entrainer concentration (ethanol volume fraction), and the influencing factors And the response value (hypericin content) as a function of. According to the regression equation to optimize the optimal process conditions. The optimum conditions were as follows: the volume fraction of ethanol was 78.6%, the extraction temperature was 50.3 ℃ and the extraction pressure was 25.29 MPa. Under these conditions, the ethanol flow rate was 0.5 mL.min-1 and the CO2 flow rate was 2 L.min- 1, the content of hypericin in the supercritical fluid extract reached 1.102% on an average of 2 h after extraction, which was much higher than that of the traditional extraction method (alkaline alcohol solution extraction), and was suitable for the extraction of hypericin from Hypericum perforatum.