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目的 探讨孕三烯酮对体外培养的子宫内膜异位症(内异症)患者的异位子宫内膜细胞(异位内膜细胞)生长及凋亡的影响,及第10号染色体缺失的磷酸酶和张力蛋白同源物(phosphataseandtensionhomologuedeletedonchromosome10,PTEN)基因在异位内膜细胞表达的变化。方法 以0、10. 6和10 .4 mol/L浓度的孕三烯酮,对体外培养的异位内膜细胞分别进行处理;采用四甲基偶氮唑蓝比色法,检测孕三烯酮对异位内膜细胞作用0 ~48h的细胞抑制情况并绘制细胞生长曲线;应用透射电镜观察孕三烯酮对异位内膜细胞作用24h时的细胞超微结构变化;采用流式细胞仪检测异位内膜细胞凋亡率及细胞周期变化;应用PTEN单克隆抗体,经流式细胞仪分析异位内膜细胞PTEN基因的表达。结果 孕三烯酮作用于异位内膜细胞8、16、24、32、40和48h的细胞生长率,在孕三烯酮浓度为10 .6 mol/L时,分别为99 .6%、87 .3%、79 .8%、62 .3%、51. 7%和44. 2%;在10 .4 mol/L时,分别为99 .2%、77 .1%、69. 6%、51 .1%、33 .7%和23 .6%。同一浓度各作用时间比较,差异均有统计学意义(P<0 .05)。孕三烯酮作用24h后,异位内膜细胞发生典型的细胞凋亡形态学改变。浓度为10 6、10 4 mol/L的孕三烯酮作用于异位内膜细胞后,凋亡率分别为1 3%、15 .0%;浓度为0mol/L的孕三烯酮?
Objective To investigate the effect of gestrinone on the growth and apoptosis of ectopic endometrial cells (ectopic endometrial cells) in patients with endometriosis (endometriosis) cultured in vitro and the effect of gestrinone on the growth and apoptosis of ectopic endometrial cells Changes of Phosphatase and Tension Homologuedeleted on Chromosome 10 (PTEN) Gene Expression in Ectopic Endometrium. Methods Ectopic endometrial cells cultured in vitro were treated with gestrinone at concentrations of 0, 10, 6 and 10. 4 mol / L, respectively. Tetramethythiazole blue colorimetric assay Ketone on ectopic endometrial cells 0 ~ 48h of cell inhibition and mapping of cell growth curve; using transmission electron microscopy gestrinone ectopic endometrial cells 24h after the change of ultrastructure; using flow cytometry The apoptosis rate and cell cycle of ectopic endometrial cells were detected. PTEN monoclonal antibody was used to analyze the expression of PTEN gene in ectopic endometrial cells by flow cytometry. Results The cell growth rate of gestrinone induced by ectopic endometrial cells at 8, 16, 24, 32, 40 and 48 hours was 99.6% at the germatrin concentration of 10.6 mol / L, 87.3%, 79.8%, 62.3%, 51.7% and 44.2%, respectively. At 10.4 mol / L, they were 99.2%, 77.1%, 69.6% , 51.1%, 33.7% and 23.6% respectively. The same concentration of each action time, the differences were statistically significant (P <0 .05). Gestrinone 24h after ectopic endometrial cells typical apoptotic morphological changes. After treated with gestrinone at a concentration of 10 6 and 10 4 mol / L for ectopic endometrial cells, the apoptotic rates were 13% and 15.0%, respectively. When the concentration of gestrinone was 0 mol / L,