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目的 探讨肿瘤抑制基因脆性组氨酸三联(FHIT)基因在上皮性卵巢癌(简称卵巢癌)组织中的甲基化状态以及该基因定位的3p14位点的等位基因丢失及其在卵巢癌的发生发展中的作用。方法 采用甲基化特异性聚合酶链反应(MSP)方法检测61例卵巢癌组织及10例交界性上皮性卵巢肿瘤(简称交界性卵巢肿瘤)组织FHIT基因启动子CpG岛的甲基化频率,并采用微卫星多态性标志D3S1287检测45例卵巢癌组织3p14位点杂合子丢失(LOH)和纯合子丢失(HD)状态。结果 卵巢癌组织中FHIT基因甲基化频率为39 3% (24 /61),其中浆液性囊腺癌为45 2% ( 19 /42 ),黏液性囊腺癌为14 3% (1 /7),子宫内膜样癌为33 3% (4 /12);交界性卵巢肿瘤组织中FHIT基因甲基化为6 /10,其中交界性浆液性囊腺瘤1 /3,交界性黏液性囊腺瘤5 /7。卵巢癌组织甲基化频率与临床分期、细胞分化程度相关性无统计学意义。交界性卵巢肿瘤与卵巢癌之甲基化频率差异无统计学意义。43 5% (10 /23)卵巢癌显示LOH;有信息的浆液性囊腺癌中33 3% (6 /18)检测到HD。FHIT基因甲基化与基因丢失之间无明显相关性。结论 首次证实卵巢癌FHIT基因启动子有较高的甲基化频率,这可能是FHIT基因沉默的重要原因,在卵巢癌的发生与发展过程中起着重要的作用;同时3p14位点等位基因的丢失可使基因?
Objective To investigate the methylation status of the tumor suppressor gene FHIT gene in epithelial ovarian cancer (ovarian cancer) tissues and its allelic loss at 3p14 and its significance in ovarian cancer The development of the role. Methods Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation frequency of FHIT gene promoter CpG island in 61 cases of ovarian cancer tissues and 10 cases of borderline ovarian tumors (borderline ovarian tumors) The heterozygous loss (LOH) and homozygous loss (HD) status of 3p14 locus in 45 cases of ovarian cancer tissues were detected by microsatellite polymorphism marker D3S1287. Results The methylation frequency of FHIT gene was 39.3% (24/61) in ovarian cancer tissues, 45.2% (19/42) in serous cystadenocarcinoma and 14.3% (1/7) in mucinous cystadenocarcinoma ). The endometrioid carcinoma was 33 3% (4/12). The methylation of FHIT gene in borderline ovarian tumor tissues was 6/10, including borderline serous cystadenoma 1/3, borderline mucinous cyst Adenoma 5/7. Ovarian cancer methylation frequency and clinical stage, the degree of cell differentiation was not related to statistical significance. There was no significant difference in methylation frequency between borderline ovarian tumor and ovarian cancer. 43 5% (10 of 23) of ovarian cancers showed LOH; 33 3% (6 of 18) of the informative serous cystadenocarcinomas detected HD. There was no significant correlation between FHIT gene methylation and gene loss. Conclusions The high methylation frequency of FHIT gene promoter in ovarian cancer was confirmed for the first time. This may be an important reason for FHIT gene silencing and plays an important role in the occurrence and development of ovarian cancer. Meanwhile, the 3p14 allele The loss can make genes?