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为进一步研究扇贝多肽 (海洋肽 ,PCF)对淋巴细胞的作用及其成分。采用地塞米松 ( DEX)与脾脏和胸腺淋巴细胞共同培养 ,建立了淋巴细胞免疫抑制实验模型。并利用反相液相色谱法 ( RPLC)对 PCF进行了分析。结果表明 ,DEX能显著降低脾脏和胸腺淋巴细胞的活性 ,DEX组吸收度值明显降低 ( P<0 .0 1) ;而 PCF不仅能显著地减轻其对免疫细胞的抑制作用 ,同时还可以促进免疫细胞的活性 ,使 DEX+PCF组吸收度值明显增高( P<0 .0 1)。相对分子质量为 80 0～ 10 0 0 D的 PCF经 RPL C分析含有保留时间为 3.92 3,4 .865,5.0 33,5.4 4 1等4个组分。实验证明 ,PCF对 DEX引起的淋巴细胞抑制具有保护作用。
To further study the scallop polypeptide (marine peptide, PCF) on lymphocytes and its components. Using dexamethasone (DEX) and spleen and thymus lymphocyte co-culture, established an experimental model of lymphocyte immunosuppression. PCF was analyzed by reversed-phase liquid chromatography (RPLC). The results showed that DEX can significantly reduce the activity of lymphocytes in spleen and thymus, the DEX group significantly decreased the absorbance value (P <0.01), while PCF can not only significantly reduce its inhibitory effect on immune cells, but also can promote Immune cell activity, the DEX + PCF group significantly increased the absorbance value (P <0.01). PCF with relative molecular mass of 80 0 ~ 100 D contained 4 components such as 3.92 3,4. 865,5.0 33,5.4 4 1 after RPL C analysis. Experiments show that PCF has a protective effect on DEX-induced lymphocyte suppression.