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目的:观察脑还丹对APP/PS1双转基因小鼠学习记忆功能及海马区细胞内钙离子浓度的影响。方法:将3月龄APP/PS1双转基因小鼠(雄性)36只,随机分为模型组、脑还丹组和多奈哌齐组,每组各12只,同月龄同遗传背景C57BL/6J小鼠12只为正常对照组。治疗组分别以脑还丹浓缩液及多奈哌齐灌胃;正常对照组和模型组以同体积蒸馏水灌胃,5月后进行行为学测试,并测定各种小鼠海马组织细胞[Ca2+]i。结果:Morris水迷宫实验:与模型组相比,正常对照组、脑还丹组和多奈哌齐组逃避潜伏期较模型组明显缩短(P<0.05),药物治疗组在原平台象限停留时间明显增加(P<0.01,P<0.05)。海马区[Ca2+]i:模型组较对照组海马细胞[Ca2+]i明显增高(P<0.01);脑还丹组及多奈哌齐组[Ca2+]i较模型组明显降低(P<0.01);而脑还丹组和多奈哌齐组之间无明显差异(P>0.05)。结论:脑还丹对APP/PS1双转基因小鼠学习记忆功能具有改善作用,这可能与其稳定海马区细胞内[Ca2+]i,减少自由基的产生,保护细胞生物膜。
Objective: To observe the effects of Naoxu Dan on learning and memory abilities and intracellular calcium concentration in APP / PS1 double transgenic mice. Methods: Thirty-six 3-month-old APP / PS1 transgenic mice (male) were randomly divided into model group, nandandan group and donepezil group, 12 in each group. The same age and same age as C57BL / 6J mice 12 Only for the normal control group. The treatment group were given Naoban Dan concentrated liquid and donepezil orally; normal control group and model group were given the same volume of distilled water, behavioral tests were performed after May, and the [Ca2 +] i of hippocampus was determined. Results: Morris water maze test: compared with the model group, the escape latency of the normal control group, the nambutan group and the donepezil group was significantly shorter than that of the model group (P <0.05), and the retention time of the drug treatment group in the original platform quadrant increased significantly (P < 0.01, P <0.05). Compared with model group, the expression of [Ca2 +] i in hippocampus was significantly lower than that in model group (P <0.01); while the ratio of [Ca2 +] i in hippocampus to model group was significantly higher than that in control group There was no significant difference between nordantex and donepezil group (P> 0.05). Conclusion: Naohuan Dan can ameliorate the learning and memory function of APP / PS1 double transgenic mice, which may be related to stabilizing [Ca2 +] i in the hippocampal area, reducing the production of free radicals and protecting cell biofilms.