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目的：确定恶性骨肿瘤（成骨肉瘤）的热疗最佳温度与时间．方法：采用临床新鲜成骨肉瘤标本20例与成骨肉瘤培养细胞，不同温度不同时间作用后．观察细胞活性，进行电镜、光镜、免疫组化检查．结果：45℃作用30min85％的成骨肉瘤细胞死亡，50℃作用30min全部肿瘤细胞即时死亡，电镜下组织标本呈现不可逆性破坏，免疫组化抗原大部分保存完好，大于50℃则抗原明显减弱，大于70℃则抗原逐渐消失．结论：50℃30min可作为成骨肉瘤热疗温度、时间．
Objective: To determine the optimal temperature and time for hyperthermia in bone tumors (osteogenesis sarcoma). METHODS: Twenty patients with clinical osteosarcoma sarcoma were treated with osteogenic sarcoma cultured cells at different temperatures and different times. The activity of the cells was observed and examined by electron microscopy, light microscopy, and immunohistochemistry. RESULTS: Osteosarcoma cells were killed at 45°C for 30 min and 80% of the osteosarcoma cells died. Immediately after exposure to 50°C for 30 min, all tumor cells died. Under electron microscope, the tissue specimens showed irreversible damage. Most of the immunohistochemical antigens were preserved well, and antigens were significantly weakened when they were greater than 50°C. More than 70 °C the antigen gradually disappeared. Conclusion: The temperature and time of hyperthermia of osteosarcoma can be treated at 50°C for 30min.