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目的：研究反义 Ｉ Ｇ ＦⅠ Ｒ 基因对肝癌细胞株７７２１ 细胞生长的抑制作用。方法：利用基因治疗的反义技术，通过脂质体介导将 Ｉ Ｇ ＦⅠ Ｒ正、反义基因真核表达载体导入人肝癌细胞株 Ｓ Ｍ Ｍ Ｃ７７２１，经潮霉素筛选阳性克隆后， 分别采用流式细胞仪检测细胞周期、凋亡， Ｍ Ｔ Ｔ 法检测细胞生长及软琼脂克隆形成试验。结果： Ｉ Ｇ ＦⅠ Ｒ反义细胞与７７２１ 细胞 Ｉ Ｇ ＦⅠ Ｒ正义细胞在前６ ｄ 生长趋势无明显变化，第７ 天后反义 Ｉ Ｇ ＦⅠ Ｒ 细胞生长减慢。反义 Ｉ Ｇ ＦⅠ Ｒ 基因细胞 Ｇ１／ Ｇ２ 期细胞明显增加，为６３．９％ ，明显高于７７２１ 细胞（４９．８％ ）； Ｓ期减少（１９．３％ ），细胞凋亡增加（５．８９％ ），不能在软琼脂中生长，而７７２１ 细胞及 Ｉ Ｇ ＦⅠ Ｒ 正义细胞在软琼脂中生长良好。结论：反义 Ｉ Ｇ ＦⅠ Ｒ基因对肝癌细胞株７７２１ 生长具有明显抑制作用。
Objective: To study the inhibitory effect of antisense IG F I R gene on the growth of hepatoma cell line 7721 cells. METHODS: The antisense technology of gene therapy was used to introduce the eukaryotic expression vector of the positive and antisense gene of I G F I R into the human hepatocellular carcinoma cell line S M M C7721 by liposome-mediated transfection. After cloning, cell cycle and apoptosis were detected by flow cytometry, and cell growth and soft agar colony formation assay were detected by MTT assay. RESULTS: There was no significant change in the growth trend of IGF-IR antisense cells and 7721 cells IGF-IR positive cells in the first 6 days. After the 7th day, antisense IGF-IR cells slowed down. Antisense I G F I R gene cells significantly increased in G1/G2 cells, which was 63.9%, significantly higher than 7721 cells (49.8%); S phase decreased (19.3%), apoptosis increased (5.89%) cannot grow in soft agar, whereas 7721 cells and IGF-IR cells grow well in soft agar. Conclusion: The antisense IG F I R gene significantly inhibited the growth of hepatoma cell line 7721.