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为了探索兰花舌瓣形成的分子机制,本研究利用消减抑制杂交技术构建了莲瓣王(Cymbidium lianpanTang et Wang)捧瓣与舌瓣间的cDNA文库。对正向文库中随机挑选的147个阳性克隆测序,组装、拼接,找到37个唯一序列,其中包含25个单一序列和12个拼接序列。经同源性比对,这些EST分别归类于蛋白质折叠,结合、未知功能、应激反应、光合作用、转录调控、信号传导、生物合成、新陈代谢、MADS等10类。通过对MADS-box基因的系统进化树分析,可初步推测MADS-Contig1和MADS-Contig2属于A类基因中的SQUA-like亚家族基因。
In order to explore the molecular mechanism of the formation of orchid flap, we constructed a cDNA library between the flap and the flap of Cymbidium lianpan Tang et Wang by subtractive inhibition hybridization. 147 positive clones randomly selected in the forward library were sequenced, assembled and spliced to find 37 unique sequences, which contained 25 single sequences and 12 splicing sequences. These homologous ESTs are classified into 10 categories such as protein folding, binding, unknown function, stress response, photosynthesis, transcription regulation, signal transduction, biosynthesis, metabolism, MADS and so on. Through phylogenetic tree analysis of MADS-box gene, it can be preliminarily speculated that MADS-Contig1 and MADS-Contig2 belong to the SQUA-like subfamily of A-type genes.