为了研究ｐ５３基因突变与人膀胱肿瘤之间的关系，应用免疫组化结合图像分析对５８例膀胱肿瘤标本进行检测。结果发现突变后的ｐ５３蛋白阳性率为２７．６％。显色主要定位在细胞核上（８７．５％），少部分在胞浆内（１２．５％）。它的阳性率在肿瘤的病理分级，临床分期及预后等方面差异显著（Ｐ＜０．０５）。结果提示，ｐ５３基因突变与人膀胱肿瘤生物学行为之间关系密切，是判断膀胱肿瘤恶性程度及预后的一项重要指标。 In order to study the relationship between p53 gene mutation and human bladder tumor, 58 cases of bladder tumor were examined by immunohistochemistry and image analysis. The results showed that the mutation rate of p53 protein was 27.6%. Coloring mainly located in the nucleus (87.5%), a small part of the cytoplasm (12.5%). Its positive rate in tumor pathological grading, clinical stage and prognosis were significantly different (P <0.05). The results suggest that the mutation of p53 gene is closely related to the biological behavior of human bladder tumor, which is an important index to judge the malignant degree and prognosis of bladder tumor.