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[目的]探讨宫颈癌、原位癌及正常宫颈组织中PTEN和TGF-β1蛋白的表达及意义。[方法]采用免疫组化Envision法检测宫颈癌组织56例、原位癌24例、正常宫颈组织20例中PTEN和TGF-β1的表达水平。[结果]宫颈癌组织中的PTEN和TGF-β表达明显低于原位癌和正常宫颈组织,差异有统计学意义(χ2=18.76,P﹤0.01;χ2=15.04,P﹤0.01),PTEN在宫颈原位癌、宫颈鳞癌Ⅰ～Ⅱ期和宫颈鳞癌Ⅲ期中的表达差异有统计学意义(P﹤0.05),在宫颈癌高分化和中低分化程度与淋巴结转移间差异都有统计学意义(P﹤0.05);TGF-β1在宫颈原位癌、宫颈鳞癌Ⅰ～Ⅱ期和宫颈鳞癌Ⅲ期中以及宫颈癌高、中低分化程度间差异无统计学意义,在有无淋巴结转移间差异有统计学意义(P﹤0.05)。[结论]PTEN蛋白的表达缺失与宫颈癌的发生、发展过程有关,TGF-β1表达与宫颈癌的侵袭转移有关。检测宫颈癌中PTEN和TGF-β1蛋白表达对宫颈癌早期诊断和判断预后有一定价值。
[Objective] To investigate the expression and significance of PTEN and TGF-β1 in cervical carcinoma, carcinoma in situ and normal cervical tissue. [Methods] Immunohistochemical Envision method was used to detect the expression of PTEN and TGF-β1 in 56 cases of cervical cancer, 24 cases of carcinoma in situ and 20 cases of normal cervical tissue. [Results] The expressions of PTEN and TGF-β in cervical cancer tissues were significantly lower than those in situ carcinoma and normal cervical tissues (χ2 = 18.76, P <0.01; χ2 = 15.04, P <0.01) Cervical carcinoma in situ, cervical squamous cell carcinoma Ⅰ ~ Ⅱ and cervical squamous cell carcinoma Ⅲ in the expression of the difference was statistically significant (P <0.05), in cervical cancer with poorly differentiated and moderately differentiated lymph node metastasis were statistically significant (P <0.05). There was no significant difference in the expression of TGF-β1 in cervical carcinoma in situ, cervical squamous cell carcinoma Ⅰ ~ Ⅱ and cervical squamous cell carcinoma Ⅲ, as well as cervical cancer with high and moderate differentiation, with or without lymph node metastasis The difference was statistically significant (P <0.05). [Conclusion] The loss of PTEN expression is related to the occurrence and development of cervical cancer. The expression of TGF-β1 is related to the invasion and metastasis of cervical cancer. Detection of cervical cancer in the expression of PTEN and TGF-β1 cervical cancer early diagnosis and prognosis of a certain value.