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目的:为提高罗汉果甜甙生产效率和产品的质量(纯度),利用闪式提取法改进传统超声波法和微波法提取罗汉果甜甙的生产工艺。方法:通过正交试验优选罗汉果甜甙最佳分离提取工艺,并利用高效液相(HPLC)检测方法,检定纯化后的甜甙样品纯度。结果:最佳闪式提取条件:物料加18倍量水,温度40℃,提取时间7 min甜甙最佳提取率为8.77%;获得的罗汉果甜甙的纯度为92%以上。结论:建立了高效生产罗汉果甜甙的新工艺。
Objective: To improve the production efficiency and product quality (purity) of Luo Han Guo Tian glycosides by flash extraction method to improve the traditional ultrasonic method and microwave extraction of mogrosides production technology. Methods: The optimal extraction and extraction technology of mogroside was optimized by orthogonal test. The purity of the purified mogroside sample was tested by high performance liquid chromatography (HPLC). Results: The best conditions of flash extraction were as follows: the material was added with 18 times of water, the temperature was 40 ℃, the optimal extraction rate of the glycosides was 8.77% at the extraction time of 7 min; the purity of the obtained glycosides was above 92%. Conclusion: A new process for the efficient production of mogrosides was established.