研究了内皮细胞在纳米和微米丝素支架上的细胞应答,包括细胞形态、增殖、细胞间连接的形成以及整合素依赖性粘附的情况。在纳米纤维网上培养的内皮细胞能够形成具有可分化性和相互连接的内皮单细胞层,它比在微米纤维网上的细胞具有更高的整合素-β1表达水平,内皮细胞通过整合素受体机制识别单根纳米纤维,并在与纳米纤维网的接触面形成粘附斑。纳米丝素支架不阻碍内皮细胞层的形成,而且可以诱导内皮细胞在分子表达水平上发生变化。 The cellular responses of endothelial cells to nanometer and micron silk fibroin scaffolds were investigated, including cell morphology, proliferation, formation of intercellular junctions, and integrin-dependent adhesion. Endothelial cells cultured on nanoweb can form an endothelial monolayer that is differentiated and interconnected, which has a higher level of integrin-β1 expression than cells on the microfibrous web. Endothelial cells are activated by the integrin receptor mechanism Identify single nanofibers and form adherent spots at the interface with the nanoweb. Nano-silk fibroin scaffold does not hinder the formation of endothelial cell layer, but also can induce changes in the level of molecular expression of endothelial cells.