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本文就应用PCR 技术对慢活肝(CAH)患者的血清HBV-DNA 检测的临床意义做一些探讨。一、材料与方法1.检测对象:我院住院的慢活肝患者75例,转氨酶反复异常达1年以上,男52例,女22例,年龄14～64岁,平均38.3岁。临床诊断标准按1990年上海《全国病毒性肝炎防治方案》所制定标准,其中有12例肝穿证实。HBV-M 均阴性的再测其抗HAV 和抗HCV 也均为阴性。2.血清HBV-M 测定:HBsAg、抗HBs、HBeAg、抗HBe、抗HBc 测定均用ELISA 法,
In this paper, the application of PCR technology in slow-living liver (CAH) serum HBV-DNA detection of clinical significance to do some discussion. Materials and Methods 1. Subjects: 75 cases of slow-living liver patients hospitalized in our hospital, ALT repeated abnormalities for more than 1 year, 52 males and 22 females, aged 14 to 64 years old, with an average of 38.3 years old. Clinical diagnostic criteria according to the 1990 Shanghai “national viral hepatitis prevention and treatment program” set by the standard, of which 12 cases of liver wear confirmed. HBV-M were negative again tested anti-HAV and anti-HCV are also negative. Serum HBV-M determination: HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc were measured by ELISA,