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目的建立X染色体短串联重复序列DXS7133、GATA198A10、DXS9896、DXS6797基因座的复合扩增系统,调查成都汉族人群的遗传多态性并探讨其法医学应用价值。方法应用PCR和非变性聚丙烯酰胺凝胶电泳分型技术,并检验各基因座女性基因型频率分布是否符合HardyWeinberg平衡,计算法医学常用各种概率。结果DXS7133、GATA198A10、DXS9896、DXS6797基因座在成都地区汉族群体中(男100,女120)分别发现6、6、11、8个等位基因,女性个人识别几率分别达0.7962、0.8021、0.9675和0.9444。χ2检验表明各基因座女性的基因型频率分布符合HardyWeinberg平衡。32个亲子鉴定案例调查表明这4个基因座符合X染色体伴性遗传方式,未发现突变。结论DXS7133、GATA198A10、DXS9896、DXS6797基因座在成都汉族群体中具有较高的遗传多态性,适用于个人识别和女孩的亲权鉴定。
Objective To establish a multiplex amplification system of DXS7133, GATA198A10, DXS9896 and DXS6797 loci of X chromosome short tandem repeats and investigate the genetic polymorphism of Chengdu Han population in Chengdu and to explore its value of forensic science. Methods PCR and non-denaturing polyacrylamide gel electrophoresis typing methods were used to test whether the frequency distribution of female genotypes in each locus was in accordance with Hardy Weinberg equilibrium and various commonly used probabilities of forensic medicine were calculated. Results There were 6, 6, 11 and 8 alleles of DXS7133, GATA198A10, DXS9896 and DXS6797 loci in Chengdu Han population (male 100 and female 120) respectively. The odds of individual identification in female were 0.7962, 0.8021, 0.9675 and 0.9444 . The χ2 test showed that the frequency distribution of genotypes in each locus was in accordance with Hardy Weinberg equilibrium. A total of 32 paternity test cases showed that the four loci were in accordance with the X chromosome inheritance, and no mutation was found. Conclusion The loci of DXS7133, GATA198A10, DXS9896 and DXS6797 have higher genetic polymorphisms in Chengdu Han population and are suitable for personal identification and girl parental identification.