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[目的]获得与油菜温敏雄性不育系K121S育性转换相关的功能基因。[方法]以油菜温敏雄性不育系K121S为材料,分别取可育温度下和不育温度下的花粉母细胞期、四分体期、三核期和成熟期的花蕾提取RNA,利用DDRT-PCR方法进行基因表达差异显示,共获得了44条差异表达的c DNA片段。[结果]经测序和BLAST序列比对分析,筛选出7个候选基因的差异表达与K121S的育性转换有关,推测胼胝质合成酶基因、乙醛脱氢酶基因、RNA聚合酶Ⅰ转录因子RRN3基因在转录水平差异表达,而H+-ATP酶基因、I类果糖二磷酸醛缩酶基因、富含亮氨酸重复的丝氨酸/苏氨酸类受体激酶基因、碱性/中性转化酶基因在转录后水平RNA剪接时差异表达。[结论]该研究结果对揭示油菜温敏雄性不育的分子机理提供了依据。
[Objective] The research aimed to obtain functional genes related to fertility transformation of thermo-sensitive male sterile line K121S in rapeseed. [Method] With rapeseed thermo-sensitive male sterile line K121S as material, RNA was extracted from flower buds of pollen mother cell, tetrad, trinucleus and maturity at fertile temperature and infertile temperature, respectively. -PCR method for gene expression differences showed a total of 44 differentially expressed c DNA fragments. [Result] The results of sequencing and BLAST analysis showed that the differential expression of seven candidate genes was related to the fertility transformation of K121S, suggesting that the genes of callose synthase, aldehyde dehydrogenase, RNA polymerase Ⅰ transcription factor RRN3 The genes are differentially expressed at the transcriptional level, while the H + -ATPase gene, the class I fructose diphosphate aldolase gene, the leucine-rich repeated serine / threonine receptor kinase gene, the alkaline / neutral invertase gene Differentially expressed at post-transcriptional level of RNA splicing. [Conclusion] The results of this study provided the basis for revealing the molecular mechanism of temperature-sensitive male sterile in rapeseed.