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目的:用紫外分光光度法建立稀针嗜蓝孢孔菌多糖的定量分析方法。方法:采用苯酚-硫酸法,以葡萄糖含量为指标,用L9(34)正交试验法选择最佳显色条件,检测波长为488 nm。结果:苯酚、浓硫酸二因素对多糖含量有显著影响,以葡萄糖表示,本法在(3.223~12.89)μg.mL-1范围内,吸收度与浓度有良好的线性关系,平均回收率96.60%,RSD为1.71%。结论:本法快速、简便,灵敏度、重复性好,不受蛋白质干扰,亦可用于混有糖苷的粗多糖的测定。
OBJECTIVE: To establish a quantitative method for the determination of Pleurotus punctatus polysaccharides by UV spectrophotometry. METHODS: The phenol-sulfuric acid method was used to determine the optimal conditions for color development using the L9 (34) orthogonal test with glucose content as the index. The detection wavelength was 488 nm. RESULTS: The two factors of phenol and concentrated sulfuric acid had a significant effect on the polysaccharide content. In terms of glucose, this method was in the range of (3.223 to 12.89) μg.mL-1. There was a good linear relationship between absorbance and concentration. The average recovery rate was 96.60%. RSD is 1.71%. Conclusion: This method is rapid, simple, sensitive, reproducible, free from protein interference, and can also be used for the determination of polysaccharides mixed with glycosides.