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通过16S rDNA克隆文库构建、PCR-RFLP分析等方法,对西太平洋“暖池”区海床以下230cm深度的沉积物中的细菌类群组成进行了分析.结果表明该海区深部沉积物中的细菌类群主要包括α-变形菌(Proteobacteria),β-变形菌、CFB类群(Cytophaga/Flexibacteria/Bacteroides)、酸杆菌(Acidobacteria)和革兰氏阳性菌等,各个类群的菌属组成均较为简单.其中α-变形菌为最优势菌群,β-变形菌为次优势菌群,它们的优势菌种分别为少动鞘氨醇单胞菌(Sphingomonas paucimobilis)和产碱假单胞菌(Pseudomonas alcaligenes).CFB类群中的细菌种类较为单一,主要为黄杆菌属(Flavobacterium).革兰氏阳性菌在该深度沉积物中的数量也较多,主要为地芽孢杆菌属(Geobacillus).对细菌类群的分析表明西太平洋“暖池”区该深度的沉积物中仍然存在较为丰富的有机物质,而这些深部生物圈中的细菌在“暖池”区海底沉积物环境的氮循环中可能起着重要的作用.
The bacterial community composition in 230 cm depth sediments below the seabed in the western Pacific Ocean “Warm Pool” was analyzed by 16S rDNA cloning library construction and PCR-RFLP analysis. The results showed that Bacteria mainly include Proteobacteria, β-Proteobacteria, Cytophaga / Flexibacteria / Bacteroides, Acidobacteria and Gram-positive bacteria. The genus of each group is relatively simple. Among them, α-Proteobacteria was the dominant group and β-Proteobacteria was the dominant group. Their dominant species were Sphingomonas paucimobilis and Pseudomonas alcaligenes ) .The genus of bacteria in the CFB group was single, mainly belonging to the genus Flavobacterium.The number of Gram-positive bacteria in this sediment was also large, mainly Geobacillus, Shows that abundant organic matter is still present in this deep sediment in the western Pacific Ocean and that these bacteria in the deep biosphere may be in the nitrogen cycle of the marine sediment environment in the “warm pool” It plays an important role.