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目的通过分子生物学方法,筛选新疆少数民族献血者样本中Fy(a-)、s-表型,了解这2种稀有血型抗原的分布情况,以解决Fy(a-)、s-稀有血型患者用血问题。方法采用多重PCR体系,以每5份标本混合检测方式,对1 020份无血缘关系健康少数民族献血者的血液DNA标本进行Fyb和S血型抗原基因分型。通过PCR-SSP验证方法,分别检测多重PCR中含有Fy(a-b+)/Fy(a+b+)或S+s-/S+s+阳性标本的高频等位基因Fya或s,从而获得稀有血型Fy(a-b+)或S+s-。结果在1 020例献血者中筛选出稀有血型Fy(a-)89例,s-46例。结论新疆少数民族献血人群中Fyb和S等位基因频率分别为29.66%和21.32%,通过筛选获得的稀有血型数据,为临床输注配合性血液提供了参考资料,并进一步丰富了中国稀有血型库。
Objective To screen the Fy (a -) and s-phenotypes of blood samples from ethnic minority donors in Xinjiang by molecular biology method and to find out the distribution of these two rare blood group antigens in Fy (a -), s- Use blood problems. Methods A multiplex PCR system was used to genotype Fyb and S blood group antigens in blood samples of blood samples from 1 020 blood donors with unrelated healthy ethnic minorities by mixed test method of 5 samples. High-frequency alleles Fya or s containing Fy (a-b +) / Fy (a + b +) or S + s- / S + s + positive samples in multiplex PCR were detected by PCR-SSP verification method, respectively, Blood type Fy (a-b +) or S + s-. Results A total of 1,020 blood donors were screened for 89 cases of rare type Fy (a-) and 46 cases of s-46 cases. Conclusion The allele frequencies of Fyb and S in blood donors from ethnic minority areas in Xinjiang are 29.66% and 21.32% respectively. By screening rare blood group data, this study provides a reference for clinical transfusion of matched blood and further enriches the data of rare genotypes .